• Western blot analysis of lysates from K562 cells, using GATA1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GATA1 (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GATA1-Ser142 antibody (109-158 aa) (STJ91112)

SKU:
STJ91112

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Erythroid transcription factor-Ser142 (109-158 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA1
Gene ID: 2623
Uniprot ID: GATA1_HUMAN
Immunogen Region: 109-158 aa
Specificity: Phospho-GATA-1 (S142) Polyclonal Antibody detects endogenous levels of GATA-1 protein only when phosphorylated at S142.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GATA1 around the phosphorylation site of Ser142 at the amino acid range 109-158
Post Translational Modifications Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Function Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
Protein Name Erythroid Transcription Factor
Eryf1
Gata-Binding Factor 1
Gata-1
Gf-1
Nf-E1 Dna-Binding Protein
Database Links Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Erythroid Transcription Factor antibody
Anti-Eryf1 antibody
Anti-Gata-Binding Factor 1 antibody
Anti-Gata-1 antibody
Anti-Gf-1 antibody
Anti-Nf-E1 Dna-Binding Protein antibody
Anti-GATA1 antibody
Anti-ERYF1 antibody
Anti-GF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance