• Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABAA Gamma 2 protein phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-GABRG2-Ser327 antibody (STJA0003651)

SKU:
STJA0003651-100

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-GABAA Receptor g 2-Ser327 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Gabrg2
Gene ID: 29709
Uniprot ID: GBRG2_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH.
Tissue Specificity Expressed in brain (at protein level). Expressed in lungs, in alveolar epithelium.
Post Translational Modifications Palmitoylated by ZDHHC3/GODZ.required for the accumulation of GABA(A) receptors at the postsynaptic membrane of inhibitory GABAergic synapses. Glycosylated.
Function Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Gamma-2/GABRG2-containing GABAARs are found at both synaptic and extrasynaptic sites. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity.the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. Extrasynaptic gamma-2-containing receptors contribute to the tonic GABAergic inhibition. GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response in a gamma-2 subunit-controlled manner.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Gamma-2
Gaba(A Receptor Subunit Gamma-2
Gabaar Subunit Gamma-2
Database Links Reactome: R-RNO-977443
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cell Projection
Dendrite
Cytoplasmic Vesicle Membrane
The Gamma-2 Subunits Are Present In Golgi Synapses And On The Extrasynaptic Membranes (When Associated With Alpha-1/6 And Beta-2/3 Subunits)
And In Some Of The Mossy Fiber To Granule Cell Synapses (When Associated With Alpha-6 And Beta-2/3 Subunits)
Present In Gabaergic And Glutamatergic Synapses On Granule Cells
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Gamma-2 antibody
Anti-Gaba(A Receptor Subunit Gamma-2 antibody
Anti-Gabaar Subunit Gamma-2 antibody
Anti-Gabrg2 antibody

Information sourced from Uniprot.org

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