| Host: |
Mouse |
| Applications: |
WB/IHC |
| Reactivity: |
Chicken/Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Phospho-Fascin-Ser-39 is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M315 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500IHC 1:100ICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FSCN1 |
| Gene ID: |
6624 |
| Uniprot ID: |
FSCN1_HUMAN |
| Specificity: |
The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. |
| Immunogen: |
Clone M315 was generated from a phospho-Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF. |
| Function | Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF. |
| Protein Name | Fascin55 Kda Actin-Bundling ProteinSinged-Like ProteinP55 |
| Database Links | Reactome: R-HSA-6785807 |
| Cellular Localisation | CytoplasmCytosolCell CortexCytoskeletonStress FiberCell ProjectionFilopodiumInvadopodiumMicrovillusCell JunctionColocalized With Rufy3 And F-Actin At Filipodia Of The Axonal Growth ConeColocalized With Dbn1 And F-Actin At The Transitional Domain Of The Axonal Growth Cone |
| Alternative Antibody Names | Anti-Fascin antibodyAnti-55 Kda Actin-Bundling Protein antibodyAnti-Singed-Like Protein antibodyAnti-P55 antibodyAnti-FSCN1 antibodyAnti-FAN1 antibodyAnti-HSN antibodyAnti-SNL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
