• Western blot analysis of extracts of various cell lines, using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-FoxO1/FoxO3a/FoxO4-T24/T32/T28 antibody (STJ11101029)

SKU:
STJ11101029

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOXO3
FOXO1
Gene ID: 2309
2308
Uniprot ID: FOXO3_HUMAN
FOXO1_HUMAN
Specificity: A synthetic phosphorylated peptide around T24 of human FOXO1/FOXO3A/FOXO4FOXO1 (NP_002006.2).
Immunogen Sequence: SCTWP
Tissue Specificity O43524 - Ubiquitous.
Q12778 - Expressed in umbilical endothelial cells (at protein level). Abundantly expressed in skeletal muscle and ovary, with lower expression in the heart, placenta, lung, liver, pancreas, spleen, testis and small intestine. Weakly expressed in the brain, thymus, prostate and mucosal lining of the colon.
Alternative Antibody Names Anti-Forkhead Box Protein O3 antibody
Anti-Af6q21 Protein antibody
Anti-Forkhead In Rhabdomyosarcoma-Like 1 antibody
Anti-FOXO3 antibody
Anti-FKHRL1 antibody
Anti-FOXO3A antibody
Anti-Forkhead Box Protein O1 antibody
Anti-Forkhead Box Protein O1a antibody
Anti-Forkhead In Rhabdomyosarcoma antibody
Anti-FOXO1 antibody
Anti-FKHR antibody
Anti-FOXO1A antibody

Information sourced from Uniprot.org

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