• Western blot analysis of extracts of various cell lines, using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 antibody (STJ11101029) at dilution of 1:200 (40x lens).
  • Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit polyclonal antibody (STJ11101029) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-FoxO1/FoxO3a/FoxO4-T24/T32/T28 antibody (STJ11101029)

SKU:
STJ11101029

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-FoxO1/FoxO3a/FoxO4-T24/T32/T28 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOXO1
FOXO4
2308
4303
2309
FOXO1_HUMAN
FOXO4_HUMAN
FOXO3_HUMAN
Immunogen: A synthetic phosphorylated peptide around T24 of human FOXO1/FOXO3A/FOXO4FOXO1 (NP_002006.2).
Immunogen Sequence: SCTWP
Tissue Specificity Q12778 - Ubiquitous.
P98177 - Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
O43524 - Ubiquitous.
Q12778 - Ubiquitous.
P98177 - Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
O43524 - Ubiquitous.
Alternative Antibody Names Anti-Forkhead Box Protein O1 antibody
Anti-Forkhead Box Protein O1a antibody
Anti-Forkhead In Rhabdomyosarcoma antibody
Anti-FOXO1 antibody
Anti-FKHR antibody
Anti-FOXO1A antibody
Anti-Forkhead Box Protein O4 antibody
Anti-Fork Head Domain Transcription Factor Afx1 antibody
Anti-FOXO4 antibody
Anti-AFX antibody
Anti-AFX1 antibody
Anti-MLLT7 antibody
Anti-Forkhead Box Protein O3 antibody
Anti-Af6q21 Protein antibody
Anti-Forkhead In Rhabdomyosarcoma-Like 1 antibody
Anti-FOXO3 antibody
Anti-FKHRL1 antibody
Anti-FOXO3A antibody
Anti-Forkhead Box Protein O1 antibody
Anti-Forkhead Box Protein O1a antibody
Anti-Forkhead In Rhabdomyosarcoma antibody
Anti-FOXO1 antibody
Anti-FKHR antibody
Anti-FOXO1A antibody
Anti-Forkhead Box Protein O4 antibody
Anti-Fork Head Domain Transcription Factor Afx1 antibody
Anti-FOXO4 antibody
Anti-AFX antibody
Anti-AFX1 antibody
Anti-MLLT7 antibody
Anti-Forkhead Box Protein O3 antibody
Anti-Af6q21 Protein antibody
Anti-Forkhead In Rhabdomyosarcoma-Like 1 antibody
Anti-FOXO3 antibody
Anti-FKHRL1 antibody
Anti-FOXO3A antibody

Information sourced from Uniprot.org

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