| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Flap endonuclease 1-Ser187 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FEN1 |
| Gene ID: |
2237 |
| Uniprot ID: |
FEN1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Rad2/FEN1 (Phospho-Ser187) at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human Rad2/FEN1 (Phospho-Ser187) |
| Post Translational Modifications | Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. |
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
| Protein Name | Flap Endonuclease 1Fen-1Dnase IvFlap Structure-Specific Endonuclease 1Maturation Factor 1Mf1Hfen-1 |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-162594Reactome: R-HSA-174437Reactome: R-HSA-5651801Reactome: R-HSA-5685939Reactome: R-HSA-69166 |
| Cellular Localisation | Isoform 1: NucleusNucleolusNucleusNucleoplasmResides Mostly In The Nucleoli And Relocalizes To The Nucleoplasm Upon Dna DamageIsoform Fenmit: Mitochondrion |
| Alternative Antibody Names | Anti-Flap Endonuclease 1 antibodyAnti-Fen-1 antibodyAnti-Dnase Iv antibodyAnti-Flap Structure-Specific Endonuclease 1 antibodyAnti-Maturation Factor 1 antibodyAnti-Mf1 antibodyAnti-Hfen-1 antibodyAnti-FEN1 antibodyAnti-RAD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
