• Western blot of Hela cell lysate treated with UV (~254nm) for 20 NA showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).
  • Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0 NA or 20 NA showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559.

Anti-Phospho-FANCI-Ser559 antibody (STJA0003831)

SKU:
STJA0003831-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-FANCI-Ser559 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:250
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FANCI
Gene ID: 55215
Uniprot ID: FANCI_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 conjugated to KLH of human species
Post Translational Modifications Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding. Phosphorylated in response to DNA damage by ATM and/or ATR.
Function Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
Protein Name Fanconi Anemia Group I Protein
Protein Faci
Database Links Reactome: R-HSA-6783310
Reactome: R-HSA-6796648
Cellular Localisation Nucleus
Cytoplasm
Observed In Spots Localized In Pairs On The Sister Chromatids Of Mitotic Chromosome Arms And Not Centromeres
One On Each Chromatids
These Foci Coincide With Common Fragile Sites
They Are Frequently Interlinked Through Blm-Associated Ultra-Fine Dna Bridges
Alternative Antibody Names Anti-Fanconi Anemia Group I Protein antibody
Anti-Protein Faci antibody
Anti-FANCI antibody
Anti-KIAA1794 antibody

Information sourced from Uniprot.org

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