• Western blot analysis of extracts of HeLa cells, using Phospho-FADD-S194 polyclonal antibody (STJ11101092) at 1:1000 dilution or FADD antibody (A5819). Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-FADD-S194 Rabbit polyclonal antibody (A5819). Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-FADD-S194 antibody (STJ11101092)

SKU:
STJ11101092

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-FADD-S194 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FADD
Gene ID: 8772
Uniprot ID: FADD_HUMAN
Immunogen: A synthetic phosphorylated peptide around S194 of human FADD (NP_003815.1).
Immunogen Sequence: AMSPM
Tissue Specificity Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Post Translational Modifications (Microbial infection) Glycosylated at Arg-117 by enteropathogenic E.coli protein NleB1, C.rodentium protein NleB and S.typhimurium protein Ssek1: arginine GlcNAcylation prevents recruitment of caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors.
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Protein Name Fas-Associated Death Domain Protein
Fas-Associating Death Domain-Containing Protein
Growth-Inhibiting Gene 3 Protein
Mediator Of Receptor Induced Toxicity
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-2562578
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5675482
Reactome: R-HSA-69416
Reactome: R-HSA-75157
Reactome: R-HSA-75158
Reactome: R-HSA-9013957
Reactome: R-HSA-933543
Reactome: R-HSA-9693928
Alternative Antibody Names Anti-Fas-Associated Death Domain Protein antibody
Anti-Fas-Associating Death Domain-Containing Protein antibody
Anti-Growth-Inhibiting Gene 3 Protein antibody
Anti-Mediator Of Receptor Induced Toxicity antibody
Anti-FADD antibody
Anti-MORT1 antibody
Anti-GIG3 antibody

Information sourced from Uniprot.org

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