Anti-Phospho-ERF-Thr526 antibody (492-541 aa) (STJ91289)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ERF around the phosphorylation site of Thr526 at the amino acid range 492-541
General Information
| Short Description | Rabbit polyclonal anti-Phospho-ETS domain-containing transcription factor ERF-Thr526 (492-541 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ERF |
| Gene ID | 2077 |
| Uniprot ID | ERF_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human ERF around the phosphorylation site of Thr526 at the amino acid range 492-541 |
| Immunogen Region | 492-541 aa |
| Specificity | Phospho-ERF (T526) Polyclonal Antibody detects endogenous levels of ERF protein only when phosphorylated at T526. |
Additional Info
| Post Translational Modifications | Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF. |
| Function | Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation. |
| Protein Name | Ets Domain-Containing Transcription Factor ErfEts2 Repressor FactorPe-2 |
| Database Links | Reactome: R-HSA-2559585 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Domain-Containing Transcription Factor Erf antibodyAnti-Ets2 Repressor Factor antibodyAnti-Pe-2 antibodyAnti-ERF antibody |
Information sourced from Uniprot.org