• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERF-Thr526 antibody (492-541 aa) (STJ91289)

SKU:
STJ91289

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ETS domain-containing transcription factor ERF-Thr526 (492-541 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERF
Gene ID: 2077
Uniprot ID: ERF_HUMAN
Immunogen Region: 492-541 aa
Specificity: Phospho-ERF (T526) Polyclonal Antibody detects endogenous levels of ERF protein only when phosphorylated at T526.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ERF around the phosphorylation site of Thr526 at the amino acid range 492-541
Post Translational Modifications Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
Function Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. May be important for regulating trophoblast stem cell differentiation.
Protein Name Ets Domain-Containing Transcription Factor Erf
Ets2 Repressor Factor
Pe-2
Database Links Reactome: R-HSA-2559585
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ets Domain-Containing Transcription Factor Erf antibody
Anti-Ets2 Repressor Factor antibody
Anti-Pe-2 antibody
Anti-ERF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance