| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-Ephrin type-A receptor 3 and Ephrin type-A receptor 4 and Ephrin type-A receptor 5-Tyr779/833 (746-795 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EPHA3 EPHA5 2042 2044 2043 EPHA3_HUMAN EPHA5_HUMAN EPHA4_HUMAN |
| Immunogen Region: | 746-795 aa |
| Specificity: | Phospho-EphA3/4/5 (Y779/833) Polyclonal Antibody detects endogenous levels of EphA3/4/5 protein only when phosphorylated at Y779/833. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human EPHA3/4/5 around the phosphorylation site of Tyr779/833 at the amino acid range 746-795 |
| Post Translational Modifications | Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1. |
| Function | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development. |
| Protein Name | Ephrin Type-A Receptor 3 Eph-Like Kinase 4 Ek4 Hek4 Hek Human Embryo Kinase Tyrosine-Protein Kinase Tyro4 Tyrosine-Protein Kinase Receptor Etk1 Eph-Like Tyrosine Kinase 1 |
| Database Links | Reactome: R-HSA-2682334 Reactome: R-HSA-3928663 Reactome: R-HSA-3928665 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Secreted |
| Alternative Antibody Names | Anti-Ephrin Type-A Receptor 3 antibody Anti-Eph-Like Kinase 4 antibody Anti-Ek4 antibody Anti-Hek4 antibody Anti-Hek antibody Anti-Human Embryo Kinase antibody Anti-Tyrosine-Protein Kinase Tyro4 antibody Anti-Tyrosine-Protein Kinase Receptor Etk1 antibody Anti-Eph-Like Tyrosine Kinase 1 antibody Anti-EPHA3 antibody Anti-ETK antibody Anti-ETK1 antibody Anti-HEK antibody Anti-TYRO4 antibody |
Information sourced from Uniprot.org
