• Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-EEF2K-Ser359 antibody (336-385 aa) (STJ91253)

SKU:
STJ91253

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Eukaryotic elongation factor 2 kinase-Ser359 (336-385 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2K
Gene ID: 29904
Uniprot ID: EF2K_HUMAN
Immunogen Region: 336-385 aa
Specificity: Phospho-eEF2K (S359) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S359.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eEF2K around the phosphorylation site of Ser359 at the amino acid range 336-385
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Protein Name Eukaryotic Elongation Factor 2 Kinase
Eef-2 Kinase
Eef-2k
Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase
Database Links Reactome: R-HSA-166208
Alternative Antibody Names Anti-Eukaryotic Elongation Factor 2 Kinase antibody
Anti-Eef-2 Kinase antibody
Anti-Eef-2k antibody
Anti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibody
Anti-EEF2K antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance