• Western blot analysis of lysates from NIH/3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HUVEC cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-EEF2-Thr56 antibody (31-80 aa) (STJ91030)

SKU:
STJ91030

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Elongation factor 2-Thr56 (31-80 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2
Gene ID: 1938
Uniprot ID: EF2_HUMAN
Immunogen Region: 31-80 aa
Specificity: Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eEF2 around the phosphorylation site of Thr56 at the amino acid range 31-80
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Protein Name Elongation Factor 2
Ef-2
Database Links Reactome: R-HSA-156902
Reactome: R-HSA-5336415
Reactome: R-HSA-5358493
Reactome: R-HSA-6798695
Reactome: R-HSA-8876725
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product
Alternative Antibody Names Anti-Elongation Factor 2 antibody
Anti-Ef-2 antibody
Anti-EEF2 antibody
Anti-EF2 antibody

Information sourced from Uniprot.org

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