| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Dematin-Ser403 (356-405 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DMTN |
| Gene ID: |
2039 |
| Uniprot ID: |
DEMA_HUMAN |
| Immunogen Region: |
356-405 aa |
| Specificity: |
Phospho-Dematin (S403) Polyclonal Antibody detects endogenous levels of Dematin protein only when phosphorylated at S403. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Dematin around the phosphorylation site of Ser403 at the amino acid range 356-405 |
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity. The N-terminus is blocked. |
| Function | Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Also plays a role as a modulator of actin dynamics in fibroblasts.acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation. |
| Protein Name | DematinDematin Actin-Binding ProteinErythrocyte Membrane Protein Band 4.9 |
| Database Links | Reactome: R-HSA-5223345 |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionCytoskeletonCell MembraneMembraneEndomembrane SystemCell ProjectionLocalized At The Spectrin-Actin Junction Of Erythrocyte Plasma MembraneLocalized To Intracellular Membranes And The Cytoskeletal NetworkLocalized At Intracellular Membrane-Bounded Organelle Compartment In Platelets That Likely Represent The Dense Tubular Network MembraneDetected At The Cell Membrane And At The Parasitophorous Vacuole In Malaria-Infected Erythrocytes At Late Stages Of Plasmodium Berghei Or Falciparum Development |
| Alternative Antibody Names | Anti-Dematin antibodyAnti-Dematin Actin-Binding Protein antibodyAnti-Erythrocyte Membrane Protein Band 4.9 antibodyAnti-DMTN antibodyAnti-DMT antibodyAnti-EPB49 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
