• Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.

Anti-Phospho-Connexin 35/36-Ser276 antibody (STJA0003859)

SKU:
STJA0003859-100

£442.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Fish/Mouse/Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Connexin 35/36-Ser276 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: Antigen affinity purified from pooled serum
Dilution Range: WB 1:1000
IHC 1:500
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser276 of perch Cx35 conjugated to KLH
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance