• Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa Alpha- and the ~60 kDa Beta-CaM Kinase II phosphorylated at Thr306 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 minutes).

Anti-Phospho-CAMK2A/B-Thr306 antibody (STJA0003590)

SKU:
STJA0003590-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CaM Kinase II-Thr306 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACADM
Gene ID: 34
Uniprot ID: ACADM_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 conjugated to KLH.
Post Translational Modifications Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3.
Function Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.
Protein Name Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Mcad
Medium Chain Acyl-Coa Dehydrogenase
Mcadh
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-77288
Reactome: R-HSA-77346
Reactome: R-HSA-77348
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Mcad antibody
Anti-Medium Chain Acyl-Coa Dehydrogenase antibody
Anti-Mcadh antibody
Anti-ACADM antibody

Information sourced from Uniprot.org

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