| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-CAD protein-Ser1859 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CAD |
| Gene ID: |
790 |
| Uniprot ID: |
PYR1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human Mouse Rat CAD (phospho-Ser1859) |
| Immunogen: |
Synthesized phosho peptide around human CAD (Ser1859) |
| Function | Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase.EC 6.3.5.5), aspartate transcarbamylase (ATCase.EC 2.1.3.2) and dihydroorotase (DHOase.EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate. |
| Protein Name | Multifunctional Protein CadCarbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine AmidotransferaseGataseGlnase - Ammonium-Dependent Carbamoyl Phosphate SynthaseCpsCpsase - Aspartate Carbamoyltransferase - Dihydroorotase |
| Database Links | Reactome: R-HSA-500753 |
| Cellular Localisation | CytoplasmNucleusCytosolic And Unphosphorylated In Resting CellsTranslocates To The Nucleus In Response To Egf StimulationNuclear Import Promotes Optimal Cell Growth |
| Alternative Antibody Names | Anti-Multifunctional Protein Cad antibodyAnti-Carbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine Amidotransferase antibodyAnti-Gatase antibodyAnti-Glnase - Ammonium-Dependent Carbamoyl Phosphate Synthase antibodyAnti-Cps antibodyAnti-Cpsase - Aspartate Carbamoyltransferase - Dihydroorotase antibodyAnti-CAD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
