• Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-BCL2L11-Ser69 antibody (STJ194702)

SKU:
STJ194702

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Bcl-2-like protein 11-Ser69 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L11
Gene ID: 10018
Uniprot ID: B2L11_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Bim (phospho-Ser69)
Immunogen: Synthesized phosho peptide around human Bim (Ser69)
Post Translational Modifications Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation. Deubiquitination catalyzed by USP27X stabilizes the protein. Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.
Function Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Protein Name Bcl-2-Like Protein 11
Bcl2-L-11
Bcl2-Interacting Mediator Of Cell Death
Database Links Reactome: R-HSA-111446
Reactome: R-HSA-111453
Reactome: R-HSA-193648
Reactome: R-HSA-6802952
Reactome: R-HSA-8862803
Reactome: R-HSA-8952158
Reactome: R-HSA-9607240
Reactome: R-HSA-9614657
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Associated With Intracytoplasmic Membranes
Isoform Bimel: Mitochondrion
Translocates From Microtubules To Mitochondria On Loss Of Cell Adherence
Isoform Biml: Mitochondrion
Isoform Bims: Mitochondrion
Isoform Bim-Alpha1: Mitochondrion
Alternative Antibody Names Anti-Bcl-2-Like Protein 11 antibody
Anti-Bcl2-L-11 antibody
Anti-Bcl2-Interacting Mediator Of Cell Death antibody
Anti-BCL2L11 antibody
Anti-BIM antibody

Information sourced from Uniprot.org

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