• Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 antibody (STJ117887) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-BCAR1-Y410 Rabbit polyclonal antibody (STJ117887) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-BCAR1-Y410 antibody (STJ117887)

SKU:
STJ117887

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-BCAR1-Y410 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAR1
Gene ID: 9564
Uniprot ID: BCAR1_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y410 of human BCAR1 (NP_055382.2).
Immunogen Sequence: GVYAV
Tissue Specificity Expressed in B-cells (at protein level). Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes.
Post Translational Modifications PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.
Function Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling.
Protein Name Breast Cancer Anti-Estrogen Resistance Protein 1
Crk-Associated Substrate
Cas Scaffolding Protein Family Member 1
P130cas
Database Links Reactome: R-HSA-186763
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Cellular Localisation Cell Junction
Focal Adhesion
Cytoplasm
Cell Projection
Axon
Unphosphorylated Form Localizes In The Cytoplasm
Localizes To Focal Adhesion Sites Following Integrin Engagement
Alternative Antibody Names Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibody
Anti-Crk-Associated Substrate antibody
Anti-Cas Scaffolding Protein Family Member 1 antibody
Anti-P130cas antibody
Anti-BCAR1 antibody
Anti-CAS antibody
Anti-CASS1 antibody
Anti-CRKAS antibody

Information sourced from Uniprot.org

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