| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-p130 Cas-Tyr-751 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:2000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Bcar1 |
| Gene ID: |
25414 |
| Uniprot ID: |
BCAR1_RAT |
| Specificity: |
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier). The antibody detects a 130 kDa* protein corresp |
| Immunogen: |
Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-657 in mouse p130 Cas. |
| Post Translational Modifications | PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway. Dephosphorylated by PTPN14 at Tyr-226. |
| Function | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling. |
| Protein Name | Breast Cancer Anti-Estrogen Resistance Protein 1Crk-Associated SubstrateP130cas |
| Database Links | Reactome: R-RNO-186763Reactome: -RNO-372708Reactome: -RNO-4420097Reactome: -RNO-8849471 |
| Cellular Localisation | Cell JunctionFocal AdhesionCytoplasmCell ProjectionAxonUnphosphorylated Form Localizes In The CytoplasmLocalizes To Focal Adhesion Sites Following Integrin Engagement |
| Alternative Antibody Names | Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibodyAnti-Crk-Associated Substrate antibodyAnti-P130cas antibodyAnti-Bcar1 antibodyAnti-Cas antibodyAnti-Crkas antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
