| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1-Ser554 (520-569 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 WB 1:500-2000 IHC 1:100-1:300 ELISA 1:10000-40000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ACAP1 |
| Gene ID: | 9744 |
| Uniprot ID: | ACAP1_HUMAN |
| Immunogen Region: | 520-569 aa |
| Specificity: | Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Centaurin-beta1 around the phosphorylation site of Ser554 at the amino acid range 520-569 |
| Post Translational Modifications | Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Function | GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Protein Name | Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 Centaurin-Beta-1 Cnt-B1 |
| Cellular Localisation | Recycling Endosome Membrane Peripheral Membrane Protein Cytoplasmic Side |
| Alternative Antibody Names | Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 antibody Anti-Centaurin-Beta-1 antibody Anti-Cnt-B1 antibody Anti-ACAP1 antibody Anti-CENTB1 antibody Anti-KIAA0050 antibody |
Information sourced from Uniprot.org