• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ACAP1-Ser554 antibody (520-569 aa) (STJ91177)

SKU:
STJ91177

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1-Ser554 (520-569 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
WB 1:500-2000
IHC 1:100-1:300
ELISA 1:10000-40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAP1
Gene ID: 9744
Uniprot ID: ACAP1_HUMAN
Immunogen Region: 520-569 aa
Specificity: Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Centaurin-beta1 around the phosphorylation site of Ser554 at the amino acid range 520-569
Post Translational Modifications Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Function GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Protein Name Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1
Centaurin-Beta-1
Cnt-B1
Cellular Localisation Recycling Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 antibody
Anti-Centaurin-Beta-1 antibody
Anti-Cnt-B1 antibody
Anti-ACAP1 antibody
Anti-CENTB1 antibody
Anti-KIAA0050 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance