• Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58 (STJA0003567, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.
  • Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-14-3-3-Ser58 antibody (STJA0003567)

SKU:
STJA0003567-100

£442.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates/Sheep/Xenopus/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-14-3-3-Ser58 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:100-1:500
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ywhab
Gene ID: 56011
Uniprot ID: 1433B_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH.
Post Translational Modifications The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Isoform Short contains a N-acetylmethionine at position 1.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Protein Name 14-3-3 Protein Beta/Alpha
Prepronerve Growth Factor Rnh-1
Protein Kinase C Inhibitor Protein 1
Kcip-1 Cleaved Into - 14-3-3 Protein Beta/Alpha - N-Terminally Processed
Database Links Reactome: R-RNO-111447
Reactome: -RNO-165159
Reactome: -RNO-166208
Reactome: -RNO-170968
Reactome: -RNO-2028269
Reactome: -RNO-392517
Reactome: -RNO-450385
Reactome: -RNO-450513
Reactome: -RNO-5625740
Reactome: -RNO-5628897
Reactome: -RNO-5673000
Reactome: -RNO-5674135
Reactome: -RNO-5675221
Reactome: -RNO-75035
Reactome: -RNO-9614399
Cellular Localisation Cytoplasm
Melanosome
Alternative Antibody Names Anti-14-3-3 Protein Beta/Alpha antibody
Anti-Prepronerve Growth Factor Rnh-1 antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 Cleaved Into - 14-3-3 Protein Beta/Alpha - N-Terminally Processed antibody
Anti-Ywhab antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance