| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PHLPP2 (1250-1323) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PHLPP2 |
| Gene ID: |
23035 |
| Uniprot ID: |
PHLP2_HUMAN |
| Immunogen Region: |
1250-1323 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1250-1323 of human PHLPP2 (NP_055835.2). |
| Immunogen Sequence: |
DSLNLIEVATEVPKRKTGYF AAPTQMEPEDQFVVPHDLEE EVKEQMKQHQDSRLEPEPHE EDRTEPPEEFDTAL |
| Tissue Specificity | In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). |
| Function | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family.specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity. |
| Protein Name | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-LikePhlpp-Like |
| Database Links | Reactome: R-HSA-199418 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinNucleusIn Colorectal Cancer TissueExpression Is Concentrated In The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2 antibodyAnti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-Like antibodyAnti-Phlpp-Like antibodyAnti-PHLPP2 antibodyAnti-KIAA0931 antibodyAnti-PHLPPL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
