• Western blot analysis of extracts of various cell lines, using PHKB antibody (STJ110321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using PHKB antibody (STJ110321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PHKB antibody (854-1093) (STJ110321)

SKU:
STJ110321

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHKB
Gene ID: 5257
Uniprot ID: KPBB_HUMAN
Immunogen Region: 854-1093
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 854-1093 of human PHKB (NP_000284.1).
Immunogen Sequence: VIHIGWIISNNPELFSGMLK IRIGWIIHAMEYELQIRGGD KPALDLYQLSPSEVKQLLLD ILQPQQNGRCWLNRRQIDGS LNRTPTGFYDRVWQILERTP NGIIVAGKHLPQQPTLSDMT MYEMNFSLLVEDTLGNIDQP QYRQIVVELLMVVSIVLERN PELEFQDKVDLDRLVKEAFN EFQKDQSRLKEIEKQDDMTS FYNTPPLGKRGTCSYLTKAV MNLLLEGEVKPNNDDPCLI
Post Translational Modifications Ser-701 is probably phosphorylated by PKA. Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Function Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
Protein Name Phosphorylase B Kinase Regulatory Subunit Beta
Phosphorylase Kinase Subunit Beta
Database Links Reactome: R-HSA-70221
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Phosphorylase B Kinase Regulatory Subunit Beta antibody
Anti-Phosphorylase Kinase Subunit Beta antibody
Anti-PHKB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance