| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PHKA1 |
| Gene ID: | 5255 |
| Uniprot ID: | KPB1_HUMAN |
| Immunogen Region: | 600-820 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1). |
| Immunogen Sequence: | QTGKLSEFLTTSCCTHLSFM DPGPEGKLYSEDYDDNYDYL ESGNWMNDYDSTSHARCGDE VARYLDHLLAHTAPHPKLAP TSQKGGLDRFQAAVQTTCDL MSLVTKAKELHVQNVHMYLP TKLFQASRPSFNLLDSPHPR QENQVPSVRVEIHLPRDQSG EVDFKALVLQLKETSSLQEQ ADILYMLYTMKGPDWNTELY NERSATVRELLTELYGKVGE I |
| Tissue Specificity | Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested. |
| Post Translational Modifications | Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated. |
| Function | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin. |
| Protein Name | Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle IsoformPhosphorylase Kinase Alpha M Subunit |
| Database Links | Reactome: R-HSA-70221 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic Side |
| Alternative Antibody Names | Anti-Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle Isoform antibodyAnti-Phosphorylase Kinase Alpha M Subunit antibodyAnti-PHKA1 antibodyAnti-PHKA antibody |
Information sourced from Uniprot.org
![Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KO Validated] NCOA2 Rabbit polyclonal antibody (STJ112318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/92369/372743/STJ112318_1__72291.1713138814.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KO Validated] NCOA2 Rabbit polyclonal antibody (STJ112318) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
