• Western blot analysis of extracts of various cell lines, using PGAM2 antibody (STJ110226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of mouse heart using PGAM2 Rabbit polyclonal antibody (STJ110226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart using PGAM2 rabbit polyclonal antibody (STJ110226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat heart using PGAM2 Rabbit polyclonal antibody (STJ110226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse heart using PGAM2 rabbit polyclonal antibody (STJ110226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PGAM2 antibody (STJ110226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-PGAM2 antibody (1-253) (STJ110226)

SKU:
STJ110226

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGAM2
Gene ID: 5224
Uniprot ID: PGAM2_HUMAN
Immunogen Region: 1-253
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human PGAM2 (NP_000281.2).
Immunogen Sequence: MATHRLVMVRHGESTWNQEN RFCGWFDAELSEKGTEEAKR GAKAIKDAKMEFDICYTSVL KRAIRTLWAILDGTDQMWLP VVRTWRLNERHYGGLTGLNK AETAAKHGEEQVKIWRRSFD IPPPPMDEKHPYYNSISKER RYAGLKPGELPTCESLKDTI ARALPFWNEEIVPQIKAGKR VLIAAHGNSLRGIVKHLEGM SDQAIMELNLPTGIPIVYEL NKELKPTKPMQFLGDEETV
Tissue Specificity Expressed in the heart and muscle. Not found in the liver and brain.
Function Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.
Protein Name Phosphoglycerate Mutase 2
Bpg-Dependent Pgam 2
Muscle-Specific Phosphoglycerate Mutase
Phosphoglycerate Mutase Isozyme M
Pgam-M
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Alternative Antibody Names Anti-Phosphoglycerate Mutase 2 antibody
Anti-Bpg-Dependent Pgam 2 antibody
Anti-Muscle-Specific Phosphoglycerate Mutase antibody
Anti-Phosphoglycerate Mutase Isozyme M antibody
Anti-Pgam-M antibody
Anti-PGAM2 antibody
Anti-PGAMM antibody

Information sourced from Uniprot.org

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