• Western blot analysis of extracts of various cell lines, using PFKP antibody (STJ11103617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PFKP antibody (STJ11103617) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using PFKP antibody (STJ11103617) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using PFKP rabbit polyclonal antibody (STJ11103617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using PFKP rabbit polyclonal antibody (STJ11103617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PFKP rabbit polyclonal antibody (STJ11103617) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PFKP antibody (336-540) (STJ11103617)

SKU:
STJ11103617

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFKP (336-540) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKP
Gene ID: 5214
Uniprot ID: PFKAP_HUMAN
Immunogen Region: 336-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 336-540 of human PFKP (NP_002618.1).
Immunogen Sequence: ATPDTPACVVSLNGNHAVRL PLMECVQMTQDVQKAMDERR FQDAVRLRGRSFAGNLNTYK RLAIKLPDDQIPKTNCNVAV INVGAPAAGMNAAVRSAVRV GIADGHRMLAIYDGFDGFAK GQIKEIGWTDVGGWTGQGGS ILGTKRVLPGKYLEEIATQM RTHSINALLIIGGFEAYLGL LELSAAREKHEEFCVPMVMV PATVS
Post Translational Modifications Phosphorylation at Ser-386 promotes interaction with ATG4B. GlcNAcylation decreases enzyme activity.
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Protein Name Atp-Dependent 6-Phosphofructokinase - Platelet Type
Atp-Pfk
Pfk-P
6-Phosphofructokinase Type C
Phosphofructo-1-Kinase Isozyme C
Pfk-C
Phosphohexokinase
Database Links Reactome: R-HSA-70171
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Atp-Dependent 6-Phosphofructokinase - Platelet Type antibody
Anti-Atp-Pfk antibody
Anti-Pfk-P antibody
Anti-6-Phosphofructokinase Type C antibody
Anti-Phosphofructo-1-Kinase Isozyme C antibody
Anti-Pfk-C antibody
Anti-Phosphohexokinase antibody
Anti-PFKP antibody
Anti-PFKF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance