Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded rat liver using perilipin-2 Rabbit polyclonal antibody (STJ11105333) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-perilipin-2 antibody (325-425) (STJ11105333)

SKU:
STJ11105333
£112.50 - £524.50
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Host: Rabbit
Applications: IHC-P/IF/ICC/ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Plin2
Gene ID: 11520
Uniprot ID: PLIN2_MOUSE
Immunogen Region: 325-425
Specificity: A synthetic peptide corresponding to a sequence within amino acids 325-425 of mouse perilipin-2 (NP_031434.3).
Immunogen Sequence: TVLVNAQGLPQNIQDQAKHL GVMAGDIYSVFRNAASFKEV SDGVLTSSKGQLQKMKESLD EVMDYFVNNTPLNWLVGPFY PQSTEVNKASLKVQQSEVKA Q
Tissue Specificity Adipose tissue specific. Expressed abundantly and preferentially in fat pads.
Post Translational Modifications Acylated.primarily with C14, C16 and C18 fatty acids. Phosphorylation at Tyr-230 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis. Polyubiquitination of Nt-acetylatable A-PLIN2 by MARCHF6 lead to degradation by 26S proteasomes.
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Protein Name Perilipin-2
Adipophilin
Adipose Differentiation-Related Protein
Adrp
Database Links
Cellular Localisation Membrane
Peripheral Membrane Protein
Lipid Droplet
Alternative Antibody Names Anti-Perilipin-2 antibody
Anti-Adipophilin antibody
Anti-Adipose Differentiation-Related Protein antibody
Anti-Adrp antibody
Anti-Plin2 antibody
Anti-Adfp antibody
Anti-Adrp antibody

Information sourced from Uniprot.org

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