| Short Description : | Rabbit polyclonal PELI3 (1-280) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PELI3 |
| Gene ID: | 246330 |
| Uniprot ID: | PELI3_HUMAN |
| Immunogen Region: | 1-280 |
| Immunogen Sequence: | MVLEGNPEVGSPRTSDLQHR GNKGSCVLSSPGEDAQPGEE PIKYGELIVLGCCEEGGEET EAQRGEVTGPRAHSCYNGCL ASGDKGRRRSRLALSRRSHA NGVKPDVMHHISTPLVSKAL SNRGQHSISYTLSRSHSVIV EYTHDSDTDMFQIGRSTENM IDFVVTDTSPGGGAAEGPSA QSTISRYACRILCDRRPPYT ARIYAAGFDASSNIFLGERA AKWRTPDGLMDGLTTNGVL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PELI3 (NP_659502.2). |
| Tissue Specificity | Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung. |
| Post Translational Modifications | Phosphorylated by IRAK1 enhancing its E3 ligase activity. |
| Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Acts as a regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling. Catalyzes 'Lys-63'-linked polyubiquitination of RIPK2 in parallel of XIAP. |
| Protein Name | E3 Ubiquitin-Protein Ligase Pellino Homolog 3Pellino-3 |
| Database Links | Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-975144 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Pellino Homolog 3 antibodyAnti-Pellino-3 antibodyAnti-PELI3 antibody |
Information sourced from Uniprot.org
