![Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186175/407867/STJ11106979_1__27112.1744894351.jpg?c=1 "Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and PDXK knockdown (KD) HeLa cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1600 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186175/407868/STJ11106979_2__82490.1744894352.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDXK knockdown (KD) HeLa cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1600 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.")
![Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1600 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186175/407869/STJ11106979_3__85634.1744894353.jpg?c=1 "Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1600 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.")
![Immunofluorescence analysis of NIH/3T3 cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) ata dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186175/407870/STJ11106979_4__40042.1744894353.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) ata dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) ata dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186175/407871/STJ11106979_5__47907.1744894354.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated]PDXK Rabbit pAb (STJ11106979) ata dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186175/407867/STJ11106979_1__27112.1744894351.jpg?c=1 "Western blot analysis of various lysates using [KD Validated]PDXK Rabbit pAb (STJ11106979) at 1:1000 dilution. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | PDXK |
| Gene ID: | 8566 |
| Uniprot ID: | PDXK_HUMAN |
| Immunogen Region: | 213-312 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 213-312 of human PDXK (NP_003672.1). |
| Immunogen Sequence: | SVVMERIRMDIRKVDAVFVG TGDLFAAMLLAWTHKHPNNL KVACEKTVSTLHHVLQRTIQ CAKAQAGEGVRPSPMQLELR MVQSKRDIEDPEIVVQATVL |
| Tissue Specificity | Ubiquitous. Highly expressed in testis. Isoform 3: In adult testis and spermatozoa. |
| Function | Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions. |
| Protein Name | Pyridoxal KinasePyridoxine Kinase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-964975 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Pyridoxal Kinase antibodyAnti-Pyridoxine Kinase antibodyAnti-PDXK antibodyAnti-C21orf124 antibodyAnti-C21orf97 antibodyAnti-PKH antibodyAnti-PNK antibodyAnti-PRED79 antibody |
Information sourced from Uniprot.org
