• Immunofluorescence analysis of U2OS cells using PDXK antibody (STJ28770). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PDXK antibody (STJ28770) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PDXK antibody (1-312) (STJ28770)

SKU:
STJ28770

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDXK (1-312) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDXK
Gene ID: 8566
Uniprot ID: PDXK_HUMAN
Immunogen Region: 1-312
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human PDXK (NP_003672.1).
Immunogen Sequence: MEEECRVLSIQSHVIRGYVG NRAATFPLQVLGFEIDAVNS VQFSNHTGYAHWKGQVLNSD ELQELYEGLRLNNMNKYDYV LTGYTRDKSFLAMVVDIVQE LKQQNPRLVYVCDPVLGDKW DGEGSMYVPEDLLPVYKEKV VPLADIITPNQFEAELLSGR KIHSQEEALRVMDMLHSMGP DTVVITSSDLPSPQGSNYLI VLGSQRRRNPAGSVVMERIR MDIRKVDAVFVGTGDLFAA
Tissue Specificity Ubiquitous. Highly expressed in testis. Isoform 3: In adult testis and spermatozoa.
Function Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
Protein Name Pyridoxal Kinase
Pyridoxine Kinase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-964975
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Pyridoxal Kinase antibody
Anti-Pyridoxine Kinase antibody
Anti-PDXK antibody
Anti-C21orf124 antibody
Anti-C21orf97 antibody
Anti-PKH antibody
Anti-PNK antibody
Anti-PRED79 antibody

Information sourced from Uniprot.org

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