• Immunofluorescence analysis of NIH/3T3 cells using PDLIM1/CLP36 Rabbit monoclonal antibody (STJ11102956) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Mouse thymus, using PDLIM1/CLP36 antibody (STJ11102956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using PDLIM1/CLP36 antibody (STJ11102956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDLIM1/CLP36 antibody (200-300) [S6MR] (STJ11102956)

SKU:
STJ11102956

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PDLIM1/CLP36 (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDLIM1
Gene ID: 9124
Uniprot ID: PDLI1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PDLIM1/CLP36 (O00151).
Immunogen Sequence: LQEKQELNEPPKQSTSFLVL QEILESEEKGDPNKPSGFRS VKAPVTKVAASIGNAQKLPM CDKCGTGIVGVFVKLRDRHR HPECYVCTDCGTNLKQKGHF F
Tissue Specificity Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.
Protein Name Pdz And Lim Domain Protein 1
C-Terminal Lim Domain Protein 1
Elfin
Lim Domain Protein Clp-36
Cellular Localisation Cytoplasm
Cytoskeleton
Myofibril
Sarcomere
Z Line
Associates With Actin Stress Fibers
Alternative Antibody Names Anti-Pdz And Lim Domain Protein 1 antibody
Anti-C-Terminal Lim Domain Protein 1 antibody
Anti-Elfin antibody
Anti-Lim Domain Protein Clp-36 antibody
Anti-PDLIM1 antibody
Anti-CLIM1 antibody
Anti-CLP36 antibody

Information sourced from Uniprot.org

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