• Western blot analysis of extracts of various cell lines, using PDHB antibody (STJ29023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (1-300) (STJ29023)

SKU:
STJ29023

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHB
Gene ID: 5162
Uniprot ID: ODPB_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHB (NP_000916.2).
Immunogen Sequence: MAAVSGLVRRPLREVSGLLK RRFHWTAPAALQVTVRDAIN QGMDEELERDEKVFLLGEEV AQYDGAYKVSRGLWKKYGDK RIIDTPISEMGFAGIAVGAA MAGLRPICEFMTFNFSMQAI DQVINSAAKTYYMSGGLQPV PIVFRGPNGASAGVAAQHSQ CFAAWYGHCPGLKVVSPWNS EDAKGLIKSAIRDNNPVVVL ENELMYGVPFEFPPEAQSKD FLIPIGKAKIERQGTHITV
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Beta - Mitochondrial
Pdhe1-B
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Reactome: R-HSA-9837999
Reactome: R-HSA-9861559
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Beta - Mitochondrial antibody
Anti-Pdhe1-B antibody
Anti-PDHB antibody
Anti-PHE1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance