| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDHA2 |
| Gene ID: | 5161 |
| Uniprot ID: | ODPAT_HUMAN |
| Immunogen Region: | 119-388 |
| Immunogen Sequence: | HGVCYTRGLSVRSILAELTG RRGGCAKGKGGSMHMYTKNF YGGNGIVGAQGPLGAGIALA CKYKGNDEICLTLYGDGAAN QGQIAEAFNMAALWKLPCVF ICENNLYGMGTSTERAAASP DYYKRGNFIPGLKVDGMDVL CVREATKFAANYCRSGKGPI LMELQTYRYHGHSMSDPGVS YRTREEIQEVRSKRDPIIIL QDRMVNSKLATVEELKEIGA EVRKEIDDAAQFATTDPEP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1). |
| Tissue Specificity | Testis. Expressed in postmeiotic spermatogenic cells. |
| Post Translational Modifications | Phosphorylation at Ser-291, Ser-293 and Ser-298 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-291, Ser-293 and Ser-298, is required for reactivation. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - MitochondrialPdhe1-A Type Ii |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial antibodyAnti-Pdhe1-A Type Ii antibodyAnti-PDHA2 antibodyAnti-PDHAL antibody |
Information sourced from Uniprot.org
