• Western blot analysis of extracts of various cell lines, using PDHA2 antibody (STJ111984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunofluorescence analysis of NIH-3T3 cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PDHA2 Polyclonal Antibody (STJ111984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PDHA2 antibody (STJ111984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PDHA2 antibody (119-388) (STJ111984)

SKU:
STJ111984

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDHA2 (119-388) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHA2
Gene ID: 5161
Uniprot ID: ODPAT_HUMAN
Immunogen Region: 119-388
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1).
Immunogen Sequence: HGVCYTRGLSVRSILAELTG RRGGCAKGKGGSMHMYTKNF YGGNGIVGAQGPLGAGIALA CKYKGNDEICLTLYGDGAAN QGQIAEAFNMAALWKLPCVF ICENNLYGMGTSTERAAASP DYYKRGNFIPGLKVDGMDVL CVREATKFAANYCRSGKGPI LMELQTYRYHGHSMSDPGVS YRTREEIQEVRSKRDPIIIL QDRMVNSKLATVEELKEIGA EVRKEIDDAAQFATTDPEP
Tissue Specificity Testis. Expressed in postmeiotic spermatogenic cells.
Post Translational Modifications Phosphorylation at Ser-291, Ser-293 and Ser-298 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-291, Ser-293 and Ser-298, is required for reactivation.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial
Pdhe1-A Type Ii
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial antibody
Anti-Pdhe1-A Type Ii antibody
Anti-PDHA2 antibody
Anti-PDHAL antibody

Information sourced from Uniprot.org

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