Anti-PDHA2 antibody (119-388) (STJ111984)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PDHA2 (119-388) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDHA2 |
| Gene ID | 5161 |
| Uniprot ID | ODPAT_HUMAN |
| Immunogen Region | 119-388 |
| Immunogen Sequence | HGVCYTRGLSVRSILAELTG RRGGCAKGKGGSMHMYTKNF YGGNGIVGAQGPLGAGIALA CKYKGNDEICLTLYGDGAAN QGQIAEAFNMAALWKLPCVF ICENNLYGMGTSTERAAASP DYYKRGNFIPGLKVDGMDVL CVREATKFAANYCRSGKGPI LMELQTYRYHGHSMSDPGVS YRTREEIQEVRSKRDPIIIL QDRMVNSKLATVEELKEIGA EVRKEIDDAAQFATTDPEP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1). |
Additional Info
| Tissue Specificity | Testis. Expressed in postmeiotic spermatogenic cells. |
| Post Translational Modifications | Phosphorylation at Ser-291, Ser-293 and Ser-298 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-291, Ser-293 and Ser-298, is required for reactivation. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - MitochondrialPdhe1-A Type Ii |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Testis-Specific Form - Mitochondrial antibodyAnti-Pdhe1-A Type Ii antibodyAnti-PDHA2 antibodyAnti-PDHAL antibody |
Information sourced from Uniprot.org