| Host: |
Goat |
| Applications: |
Pep-ELISA/WB-Trf/IHC/EIA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PDE4D (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and EIA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
PDE4D |
| Gene ID: |
5144 |
| Uniprot ID: |
PDE4D_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1 |
| Specificity: |
This antiobody is expected to recognize all reported isoforms. |
| Immunogen Sequence: |
QPEACVIDDRSPDT |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4dDpde3Pde43Camp-Specific Phosphodiesterase 4d |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4d antibodyAnti-Dpde3 antibodyAnti-Pde43 antibodyAnti-Camp-Specific Phosphodiesterase 4d antibodyAnti-PDE4D antibodyAnti-DPDE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
