Anti-PDE4A antibody (325-410) (STJ11103193)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PDE4A (325-410) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PDE4A |
| Gene ID | 5141 |
| Uniprot ID | PDE4A_HUMAN |
| Immunogen Region | 325-410 |
| Immunogen Sequence | PVPHLQPMSQITGLKKLMHS NSLNNSNIPRFGVKTDQEEL LAQELENLNKWGLNIFCVSD YAGGRSLTCIMYMIFQERDL LKKFRI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 325-410 of human PDE4A (NP_001104777.1). |
Additional Info
| Tissue Specificity | Isoform 1: Expressed in lymphoid cell subsets including CD8-positive T cells and T-helper 2 cells. Expressed in dendritic cells. Isoform 2: Highly expressed in liver, stomach, testis, thyroid and adrenal glands and at a lower extent in placenta, kidney, pancreas, ovary, uterus and skin. Expressed in myeloid cell subsets including dendritic cells, monocytes, macrophages, eosinophils and mast cells. Expressed in natural killer cells. Expressed in bronchial smooth muscle. Isoform 6: Expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7: Expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Found in specific neuronal subpopulations including cortical pyramidal neurons, horn neurons in the spinal cord and Purkinje cells in cerebellum (at protein level). |
| Post Translational Modifications | Isoform 1: Proteolytically cleaved by CASP3. Isoform 2: Phosphorylated at Ser-119 by PKA. |
| Function | Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1: Efficiently hydrolyzes cAMP. Isoform 2: Efficiently hydrolyzes cAMP. Isoform 3: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4: Efficiently hydrolyzes cAMP. Isoform 6: Efficiently hydrolyzes cAMP. Isoform 7: Efficiently hydrolyzes cAMP. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4aDpde2Pde46Camp-Specific Phosphodiesterase 4a |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Isoform 1: CytoplasmPerinuclear RegionIsoform 2: CytoplasmCell ProjectionRuffle MembraneIsoform 3: CytoplasmCytosolIsoform 4: MembranePeripheral Membrane ProteinIsoform 4 Has Propensity For Association With MembranesIsoform 6: CytoplasmIsoform 7: CytoplasmMembranePredominantly Cytosolic |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4a antibodyAnti-Dpde2 antibodyAnti-Pde46 antibodyAnti-Camp-Specific Phosphodiesterase 4a antibodyAnti-PDE4A antibodyAnti-DPDE2 antibody |
Information sourced from Uniprot.org