• Western blot analysis of extracts of various cell lines, using PDE3B antibody (STJ110746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
  • Western blot analysis of extracts of various cell lines, using PDE3B antibody (STJ110746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-PDE3B antibody (400-660) (STJ110746)

SKU:
STJ110746

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE3B (400-660) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE3B
Gene ID: 5140
Uniprot ID: PDE3B_HUMAN
Immunogen Region: 400-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human PDE3B (NP_000913.2).
Immunogen Sequence: PLTPFPGFYPCSEIEDPAEK GDRKLNKGLNRNSLPTPQLR RSSGTSGLLPVEQSSRWDRN NGKRPHQEFGISSQGCYLNG PFNSNLLTIPKQRSSSVSLT HHVGLRRAGVLSSLSPVNSS NHGPVSTGSLTNRSPIEFPD TADFLNKPSVILQRSLGNAP NTPDFYQQLRNSDSNLCNSC GHQMLKYVSTSESDGTDCCS GKSGEEENIFSKESFKLMET QQEEETEKKDSRKLFQEGD
Tissue Specificity Abundant in adipose tissues.
Post Translational Modifications Phosphorylation at Ser-295 mediates insulin-induced activation of PDE3B.
Function Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological process. Regulates angiogenesis by inhibiting the cAMP-dependent guanine nucleotide exchange factor RAPGEF3 and downstream phosphatidylinositol 3-kinase gamma-mediated signaling. Controls cardiac contractility by reducing cAMP concentration in cardiocytes.
Protein Name Cgmp-Inhibited 3' -5'-Cyclic Phosphodiesterase 3b
Cgipde1
Cgip1
Cyclic Gmp-Inhibited Phosphodiesterase B
Cgi-Pde B
Database Links Reactome: R-HSA-165160
Reactome: R-HSA-418555
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cgmp-Inhibited 3' -5'-Cyclic Phosphodiesterase 3b antibody
Anti-Cgipde1 antibody
Anti-Cgip1 antibody
Anti-Cyclic Gmp-Inhibited Phosphodiesterase B antibody
Anti-Cgi-Pde B antibody
Anti-PDE3B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance