STJ72821 (1µg/ml) staining of nuclear NIH3T3 (A) and nuclear HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PDCD4 antibody (Internal) (STJ72821)

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STJ72821-100
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Short Description :Goat polyclonal antibody anti-PDCD4 (Internal) is suitable for use in ELISA and Western Blot research applications.
Applications:Pep-ELISA/WB
Host:Goat
Reactivity:Human/Mouse/Rat/Dog/Pig/Cow
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:0.5 mg/mL
Purification:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution Range:Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 60kDa band observed in nuclear lysates of cell lines NIH3T3 and HeLa (calculated MW of 51.7kDa according to NP_055271.2). Recommended concentration: 1-3µg/ml.
Formulation:0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage Instruction:Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol:PDCD4
Gene ID:27250
Uniprot ID:PDCD4_HUMAN
Accession Number:NP_055271.2; NP_663314.1; NP_001186421.1
Immunogen Region:Internal
Immunogen Sequence:DSYKGTVDCVQAR
Specificity:This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
Post Translational Modifications Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4.
Function Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Protein Name Programmed Cell Death Protein 4
Neoplastic Transformation Inhibitor Protein
Nuclear Antigen H731-Like
Protein 197/15a
Database Links Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm
Predominantly Nuclear Under Normal Growth Conditions
And When Phosphorylated At Ser-457
Alternative Antibody Names Anti-Programmed Cell Death Protein 4 antibody
Anti-Neoplastic Transformation Inhibitor Protein antibody
Anti-Nuclear Antigen H731-Like antibody
Anti-Protein 197/15a antibody
Anti-PDCD4 antibody
Anti-H731 antibody

Information sourced from Uniprot.org

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