Home
Primary Antibodies
Monoclonal Antibodies
Anti-PCNA antibody [12D10] (STJ96933)

IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200
Immunofluorescence analysis of Rat-testis tissue. 1, PCNA monoclonal antibody (12D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Human-lung-cancer tissue. 1, PCNA monoclonal antibody (12D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PCNA antibody [12D10] (STJ96933)

SKU:

STJ96933

£46.00 - £226.00

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IF/IHC
Reactivity:	Human/Rat/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Proliferating cell nuclear antigen is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	12D10
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range:	WB 1:5000 IHC 1:200 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PCNA
Gene ID:	5111
Uniprot ID:	PCNA_HUMAN
Specificity:	The antibody detects endogenous PCNA protein.
Immunogen:	Synthetic Peptide of PCNA

Function	Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Protein Name	Proliferating Cell Nuclear Antigen Pcna Cyclin
Database Links	Reactome: R-HSA-110312 Reactome: R-HSA-110314 Reactome: R-HSA-110320 Reactome: R-HSA-1362277 Reactome: R-HSA-174411 Reactome: R-HSA-174414 Reactome: R-HSA-174417 Reactome: R-HSA-174437 Reactome: R-HSA-4615885 Reactome: R-HSA-5358565 Reactome: R-HSA-5358606 Reactome: R-HSA-5651801 Reactome: R-HSA-5655862 Reactome: R-HSA-5656121 Reactome: R-HSA-5656169 Reactome: R-HSA-5685942 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6804114 Reactome: R-HSA-69091 Reactome: R-HSA-69166 Reactome: R-HSA-69183 Reactome: R-HSA-69205 Reactome: R-HSA-8866654
Cellular Localisation	Nucleus Colocalizes With Crebbp Ep300 And Pold1 To Sites Of Dna Damage Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase Co-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S Phase Together With Apex2 Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Alternative Antibody Names	Anti-Proliferating Cell Nuclear Antigen antibody Anti-Pcna antibody Anti-Cyclin antibody Anti-PCNA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

reviews