• Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 antibody (STJ11104393). Western blot was performed from the immunoprecipitate using PEPCK/PEPCK/PCK2 antibody (STJ11104393) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using PEPCK/PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded mouse kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded rat kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PCK2 antibody (1-280) (STJ11104393)

SKU:
STJ11104393

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCK2
Gene ID: 5106
Uniprot ID: PCKGM_HUMAN
Immunogen Region: 1-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).
Immunogen Sequence: MAALYRPGLRLNWHGLSPLG WPSCRSIQTLRVLSGDLGQL PTGIRDFVEHSARLCQPEGI HICDGTEAENTATLTLLEQQ GLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTV QLPPGGARGQLGNWMSPADF QRAVDERFPGCMQGRTMYVL PFSMGPVGSPLSRIGVQLTD SAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTG QGEPVSQWPCNPEKTLIGH
Tissue Specificity Widely expressed.
Function Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis.
Protein Name Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial
Pepck-M
Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial
Mtpck2
Database Links Reactome: R-HSA-70263
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibody
Anti-Pepck-M antibody
Anti-Phosphoenolpyruvate Carboxykinase 2 - Mitochondrial antibody
Anti-Mtpck2 antibody
Anti-PCK2 antibody
Anti-PEPCK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance