| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:10-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PC |
| Gene ID: | 5091 |
| Uniprot ID: | PYC_HUMAN |
| Immunogen Region: | 879-1178 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate carboxylase (Pyruvate carboxylase (PC) ) (NP_001035806.1). |
| Immunogen Sequence: | HSMGLGSKFKEVKKAYVEAN QMLGDLIKVTPSSKIVGDLA QFMVQNGLSRAEAEAQAEEL SFPRSVVEFLQGYIGVPHGG FPEPFRSKVLKDLPRVEGRP GASLPPLDLQALEKELVDRH GEEVTPEDVLSAAMYPDVFA HFKDFTATFGPLDSLNTRLF LQGPKIAEEFEVELERGKTL HIKALAVSDLNRAGQRQVFF ELNGQLRSILVKDTQAMKEM HFHPKALKDVKGQIGAPMP |
| Post Translational Modifications | Acetylation of Lys-748 might play a role in catalytic activity regulation. |
| Function | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. |
| Protein Name | Pyruvate Carboxylase - MitochondrialPyruvic CarboxylasePcb |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-70263Reactome: R-HSA-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Carboxylase - Mitochondrial antibodyAnti-Pyruvic Carboxylase antibodyAnti-Pcb antibodyAnti-PC antibody |
Information sourced from Uniprot.org