• Western blot analysis of extracts of various cell lines, using PAX7 Rabbit monoclonal antibody (STJ11101999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PAX7 antibody (350-450) [S9MR] (STJ11101999)

SKU:
STJ11101999

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAX7
Gene ID: 5081
Uniprot ID: PAX7_HUMAN
Immunogen Region: 350-450
Specificity: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAX7 (P23759).
Immunogen Sequence: SAYGARHSFSSYSDSFMNPA APSNHMNPVSNGLSPQVMSI LGNPSAVPPQPQADFSISPL HGGLDSATSISASCSQRADS IKPGDSLPTSQAYCPPTYST T
Post Translational Modifications Acetylation at Lys-105 and Lys-139 by KAT8 is required for high-level transcription factor activity. Deacetylated by SIRT2.
Function Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Protein Name Paired Box Protein Pax-7
Hup1
Database Links Reactome: R-HSA-9834899
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-7 antibody
Anti-Hup1 antibody
Anti-PAX7 antibody
Anti-HUP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance