• Western blot analysis of extracts of various cell lines, using PAX7 Rabbit monoclonal antibody (STJ11101999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PAX7 antibody (350-450) [S9MR] (STJ11101999)

SKU:
STJ11101999

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PAX7 (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAX7
Gene ID: 5081
Uniprot ID: PAX7_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PAX7 (P23759).
Immunogen Sequence: SAYGARHSFSSYSDSFMNPA APSNHMNPVSNGLSPQVMSI LGNPSAVPPQPQADFSISPL HGGLDSATSISASCSQRADS IKPGDSLPTSQAYCPPTYST T
Function Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Protein Name Paired Box Protein Pax-7
Hup1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-7 antibody
Anti-Hup1 antibody
Anti-PAX7 antibody
Anti-HUP1 antibody

Information sourced from Uniprot.org

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