• Western blot analysis of extracts of 293T cells, using PAX7 antibody (STJ114613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat brain using PAX7 antibody (STJ114613) at dilution of 1:100 (40x lens).

Anti-PAX7 antibody (289-518) (STJ114613)

SKU:
STJ114613

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAX7 (289-518) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAX7
Gene ID: 5081
Uniprot ID: PAX7_HUMAN
Immunogen Region: 289-518
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 289-518 of human PAX7 (NP_039236.1).
Immunogen Sequence: GFPPTGMPTLPPYQLPDSTY PTTTISQDGGSTVHRPQPLP PSTMHQGGLAAAAAAADTSS AYGARHSFSSYSDSFMNPAA PSNHMNPVSNGLSPQVMSIL GNPSAVPPQPQADFSISPLH GGLDSATSISASCSQRADSI KPGDSLPTSQAYCPPTYSTT GYSVDPVAGYQYGQYGQSEC LVPWASPVPIPSPTPRASCL FMESYKVVSGWGMSISQMEK LKSSQMEQFT
Function Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Protein Name Paired Box Protein Pax-7
Hup1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-7 antibody
Anti-Hup1 antibody
Anti-PAX7 antibody
Anti-HUP1 antibody

Information sourced from Uniprot.org

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