• Immunohistochemistry analysis of paraffin-embedded human placenta using PAPD7 Rabbit polyclonal antibody (STJ112057) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PAPD7 antibody (STJ112057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PAPD7 antibody (303-542) (STJ112057)

SKU:
STJ112057

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAPD7 (303-542) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TENT4A
Gene ID: 11044
Uniprot ID: PAPD7_HUMAN
Immunogen Region: 303-542
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 303-542 of human PAPD7 (NP_008930.1).
Immunogen Sequence: GSKAHPSPGMDSRIKIKERI ATCNGEQTQNREPESPYGQR LTLSLSSPQLLSSGSSASSV SSLSGSDVDSDTPPCTTPSV YQFSLQAPAPLMAGLPTALP MPSGKPQPTTSRTLIMTTNN QTRFTIPPPTLGVAPVPCRQ AGVEGTASLKAVHHMSSPAI PSASPNPLSSPHLYHKQHNG MKLSMKGSHGHTQGGGYSSV GSGGVRPPVGNRGHHQYNRT GWRRKKHTHTRDSLPVSLS
Function Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.
Protein Name Terminal Nucleotidyltransferase 4a
Dna Polymerase Sigma
Lak-1
Non-Canonical Poly(A Rna Polymerase Papd7
Pap-Associated Domain-Containing Protein 7
Tramp-Like Complex Polyadenylate Polymerase
Terminal Guanylyltransferase
Terminal Uridylyltransferase 5
Tutase 5
Topoisomerase-Related Function Protein 4-1
Trf4-1
Database Links Reactome: R-HSA-6802952
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Excluded From Nucleolus
Weak Staining Detected In The Cytoplasm
Alternative Antibody Names Anti-Terminal Nucleotidyltransferase 4a antibody
Anti-Dna Polymerase Sigma antibody
Anti-Lak-1 antibody
Anti-Non-Canonical Poly(A Rna Polymerase Papd7 antibody
Anti-Pap-Associated Domain-Containing Protein 7 antibody
Anti-Tramp-Like Complex Polyadenylate Polymerase antibody
Anti-Terminal Guanylyltransferase antibody
Anti-Terminal Uridylyltransferase 5 antibody
Anti-Tutase 5 antibody
Anti-Topoisomerase-Related Function Protein 4-1 antibody
Anti-Trf4-1 antibody
Anti-TENT4A antibody
Anti-PAPD7 antibody
Anti-POLS antibody
Anti-TRF4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance