| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PALLD |
| Gene ID: | 23022 |
| Uniprot ID: | PALLD_HUMAN |
| Immunogen Region: | 383-672 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 383-672 of human PALLD (NP_001159582.1). |
| Immunogen Sequence: | AVNQRGRSPRSPSGHPHVRR PRSRSRDSGDENEPIQERFF RPHFLQAPGDLTVQEGKLCR MDCKVSGLPTPDLSWQLDGK PVRPDSAHKMLVRENGVHSL IIEPVTSRDAGIYTCIATNR AGQNSFSLELVVAAKEAHKP PVFIEKLQNTGVADGYPVRL ECRVLGVPPPQIFWKKENES LTHSTDRVSMHQDNHGYICL LIQGATKEDAGWYTVSAKNE AGIVSCTARLDVYTQWHQQ |
| Tissue Specificity | Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines. |
| Post Translational Modifications | Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines. Phosphorylation at Ser-1118 by PKB/AKT1 modulates cytoskeletal organization and cell motility. |
| Function | Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci. |
| Protein Name | Palladin Sih002 Sarcoma Antigen Ny-Sar-77 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Junction Focal Adhesion Myofibril Sarcomere Z Line Cell Projection Ruffle Podosome Lamellipodium Axon Growth Cone Localizes To Stress Fibers And Z Lines Preferentially Expressed In The Excitatory Presynaptic Terminals |
| Alternative Antibody Names | Anti-Palladin antibody Anti-Sih002 antibody Anti-Sarcoma Antigen Ny-Sar-77 antibody Anti-PALLD antibody Anti-KIAA0992 antibody Anti-CGI-151 antibody |
Information sourced from Uniprot.org