• Western blot analysis of lysates from Rat liver using PAH Rabbit polyclonal antibody (STJ11105611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAH antibody (110-452) (STJ11105611)

SKU:
STJ11105611

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phenylalanine-4-Hydroxylase (110-452) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAH
Gene ID: 5053
Uniprot ID: PH4H_HUMAN
Immunogen Region: 110-452
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 110-452 of human PAH (NP_000268.1).
Immunogen Sequence: SRDKKKDTVPWFPRTIQELD RFANQILSYGAELDADHPGF KDPVYRARRKQFADIAYNYR HGQPIPRVEYMEEEKKTWGT VFKTLKSLYKTHACYEYNHI FPLLEKYCGFHEDNIPQLED VSQFLQTCTGFRLRPVAGLL SSRDFLGGLAFRVFHCTQYI RHGSKPMYTPEPDICHELLG HVPLFSDRSFAQFSQEIGLA SLGAPDEYIEKLATIYWFTV EFGLCKQGDSIKAYGAGLL
Post Translational Modifications Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.
Function Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Protein Name Phenylalanine-4-Hydroxylase
Pah
Phe-4-Monooxygenase
Database Links Reactome: R-HSA-2160456
Reactome: R-HSA-8964208
Alternative Antibody Names Anti-Phenylalanine-4-Hydroxylase antibody
Anti-Pah antibody
Anti-Phe-4-Monooxygenase antibody
Anti-PAH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance