• Immunofluorescence analysis of HepG2 cells using PAH Rabbit polyclonal antibody (STJ24886) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of HepG2, using PAH antibody (STJ24886) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PAH antibody (1-290) (STJ24886)

SKU:
STJ24886

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAH (1-290) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAH
Gene ID: 5053
Uniprot ID: PH4H_HUMAN
Immunogen Region: 1-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PAH (NP_000268.1).
Immunogen Sequence: MSTAVLENPGLGRKLSDFGQ ETSYIEDNCNQNGAISLIFS LKEEVGALAKVLRLFEENDV NLTHIESRPSRLKKDEYEFF THLDKRSLPALTNIIKILRH DIGATVHELSRDKKKDTVPW FPRTIQELDRFANQILSYGA ELDADHPGFKDPVYRARRKQ FADIAYNYRHGQPIPRVEYM EEEKKTWGTVFKTLKSLYKT HACYEYNHIFPLLEKYCGFH EDNIPQLEDVSQFLQTCTG
Post Translational Modifications Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.
Function Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Protein Name Phenylalanine-4-Hydroxylase
Pah
Phe-4-Monooxygenase
Database Links Reactome: R-HSA-2160456
Reactome: R-HSA-8964208
Alternative Antibody Names Anti-Phenylalanine-4-Hydroxylase antibody
Anti-Pah antibody
Anti-Phe-4-Monooxygenase antibody
Anti-PAH antibody

Information sourced from Uniprot.org

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