| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PACSIN3 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: EB05271 (0.03µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PACSIN3 |
| Gene ID: | 29763 |
| Uniprot ID: | PACN3_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_057307.2 |
| Immunogen Sequence: | APEEDAGGEALGGS |
| Post Translational Modifications | Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). |
| Function | Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity. |
| Protein Name | Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3Sh3 Domain-Containing Protein 6511 |
| Database Links | Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideDetected At The Inner Aspect Of The Plasma Membrane In Myotubes |
| Alternative Antibody Names | Anti-Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3 antibodyAnti-Sh3 Domain-Containing Protein 6511 antibodyAnti-PACSIN3 antibody |
Information sourced from Uniprot.org